IBR 13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
A chimeric vector for efficient chromosomal modifications in Enterococcus faecalis
Autor/es:
BLANCATO, VS; MAGNI, C
Revista:
LETTERS IN APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2010 vol. 50 p. 542 - 546
ISSN:
0266-8254
Resumen:
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AIM: To
construct a chimeric vector named pBVGh for quickly generating gene
modifications in Enterococcus faecalis.
METHODS AND RESULTS: The constructed
plasmid pBVGh carries the pG(+)host replicon (a thermosensitive (TS) derivative
of pWV01), allowing a simple generation of mutants by growing colonies first at
the permissive temperature and then switching the culture to the nonpermissive
temperature. Additionally, this vector facilitates the screening of mutants by
a rapid colorimetric blue-white discrimination of plasmid-free bacteria.
CONCLUSIONS: The pBVGh vector allows a straightforward inactivation or
modification of target genes as well as a fast selection of enterococcal mutant
strains.
SIGNIFICANCE AND IMPACT OF THE STUDY: The broad range of the TS replicon
utilized in this plasmid permits the easy establishment and the efficient
generation of food-grade mutant strains in Ent. faecalis and several other
gram-positive bacteria.
