Deleted in Malignant Brain Tumor 1 (DMBT1) Expression Pattern in Normal Cervix and at Different Stages of Squamous Intraepithelial Lesions

VALERO, ANDRÉS; ROLDÁN, MARÍA LORENA; RUIZ, MARÍA FERNANDA; TEIJEIRO, JUAN MANUEL; MARQUEZ, SUSANA BEATRIZ; MARINI, PATRICIA ESTELA

The Open Biomarkers Journal

BENTHAM OPEN

Año: 2018 vol. 8 p. 1 - 8

1875-3183

Background:Cervical cancer is one of the most frequently occurring malignancies in women worldwide, with high mortality rates. CervicalSquamous Cell Carcinoma (SCC) presents previous states of non-invasive precursor lesions, and early stage Low-Grade SquamousIntraepithelial Lesions (LSIL) regress to normal or Atypical Squamous Cells of Undetermined Significance (ASCUS) inapproximately 50% of cases. Deleted in Malignant Brain Tumors 1 (DMBT1) is a tumour suppression glycoprotein, which absence isconsidered a malignancy marker in many epithelial cancers.Objective:To analyse DMBT1?s presence and localization in SCC and precursor lesions.Method:Immunohistochemistry for DMBT1 was performed in cervix biopsies classified as normal, LSIL, HSIL and SCC.Results:DMBT1 was detected at the supranuclear and sometimes infranuclear regions of the endocervix monolayer epithelial cells in normaland HSIL biopsies. In LSIL samples the detection of DMBT1 in endocervix was variable between patients. Also variable wasDMBT1 staining in cells of glandular epithelium. The glycoprotein was not detected in the stratified epithelium of the exocervix,regardless of the lesion grade; nor in dysplastic cells.Conclusion:The absence of DMBT1 from endocervix only in some samples of LSIL is promising as a candidate for possible lesion regressionpotential marker.