DLG1 polarity protein expression associates with the disease progress of low-grade cervical intraepithelial lesions

ANA LAURA CAVATORTA A, ALEJANDRA DI GREGORIO B, MARINA BUGNON VALDANO A, FEDERICO MARZIALI A, MARIELA CABRAL B, HEBE BOTTAI C, JORGE CITTADINI B, ANA LIA NOCITO D, DANIELA GARDIOL

EXPERIMENTAL AND MOLECULAR PATHOLOGY.

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2016 vol. 102 p. 65 - 69

0014-4800

Human Discs large tumour suppressor (DLG1) participates in regulating cell polarity and proliferation, suggestingan important connection between epithelial organization and cellular growth control. However, it was demonstrated that DLG1 could acquire oncogenic attributes in some speci fic contexts. In this work, we evaluated the expression of DLG1 and its contribution to the progress of cervical lesions in order to investigate a potential role ofthis polarity protein in human oncogenic processes.We analyzed cervical biopsies from women with low-grade squamous intraepithelial lesion (LSIL) diagnosis(n = 30), for DLG1 expression by immunohistochemistry. These results were correlated with the clinical monitoring of the patients during a 24-month follow-up period. Our data indicate that while all LSIL patients with aDLG1 staining pattern similar to normal tissues are signi ficantly more likely to regress (n = 23, Pattern I), allLSIL biopsy specimens showing a diffuse and intense DLG1 staining likely progress to high-grade lesions (n =4, Pattern II). Finally, all persistent LSIL analyzed showed an undetermined DLG1 staining, with a diffuse distribution without a strong intensity (n = 3, Pattern III). We found a signi ficant association between the expression pattern of DLG1 and the evolution of the lesion (p b 0.00001).This work contributes to the knowledge of DLG1 biological functions, suggesting that its expression may have animportant role in the progression of early dysplastic cervical lesions, giving prognostic information.