IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Transcriptional Regulation of the Citrate gene cluster of Enterococcus faecalis involves the GntR family transcriptional activator CitO
Autor/es:
BLANCATO V. S.; REPIZO G. D.; SUÁREZ C. A.; MAGNI C.
Revista:
journal of bacteriology
Editorial:
ASM
Referencias:
Año: 2008 vol. 190 p. 7419 - 7430
Resumen:
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This complex splits citrate into oxaloacetate and acetate and is involved in all the known anaerobic bacterial citrate fermentation pathways. Although citrate fermentation in E. faecalis has been investigated before, the regulation and transcriptional pattern of the cit locus has still not been fully explored. To fill this gap, in this paper we demonstrate that the GntR transcriptional regulator CitO is a novel positive regulator involved in the expression of the cit operons. The transcriptional analysis of the cit clusters revealed two divergent operons: citHO, which codifies for the transporter (citH) and the regulatory protein (citO), and upstream from it and in opposite direction the oadHDBcitCDEFXoadAcitMG operon, which includes the citrate lyase subunits (citD, citE, and citF), the soluble oxaloacetate decarboxylase (citM) and also the genes encoding a putative oxaloacetate decarboxylase complex (oadB, oadA, oadD and oadH). This analysis also showed that both operons are specifically activated by the addition of citrate to the medium. In order to study the functional role of CitO, a mutant strain with an interrupted citO gene was constructed, causing a total loss of the ability to degrade citrate. Reintroduction of a functional copy of citO to the citO-deficient strain restored the response to citrate and the Cit+ phenotype. Furthermore, we present evidence that CitO binds to the cis-acting sequences O1 and O2, located in the cit intergenic region, increasing its affinity for these binding sites when citrate is present, and allowing the induction of both cit promoters