IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Insulin-Like Growth Factor-I of Pejerrey, Odontesthes bonariensis: cDNA Characterization, Tissue Distribution and Expression Profiles After Growth Hormone Administration
Autor/es:
ANDRES A. SCIARA, GUSTAVO M. SOMOZA, SILVIA E. ARRANZ
Revista:
JOURNAL OF EXPERIMENTAL ZOOLOGY
Editorial:
Wiley-Liss, Inc.
Referencias:
Lugar: Wilmington, EE.UU.; Año: 2008 vol. 309 p. 407 - 418
ISSN:
0022-104X
Resumen:
The liver production of the insulin-like growth factor-I (IGF-I) is a key factor inthe endocrine control of body growth by a growth hormone. As pejerrey Odontesthes bonariensis has been reported as a fish with low growth rates in captivity, basic research on this respect is needed in order to understand it. In this context, the pejerrey IGF-I cDNA was cloned and its hepatic expression was examined in fish after recombinant pejerrey growth hormone (pjGHr) administration. The full length of IGF-I transcript showed a high sequence similarity to other teleost sequences. The tissue distribution analysis by reverse transcriptase polymerase chain reaction in adult fish revealed that IGF-I expressed ubiquitously with the highest mRNA levels in the liver, posterior intestine and brain. No alternative IGF-I mRNA was found in the liver, as it was reported for other teleosts. IGF-I transcript was measured in the liver after pjGHr in vivo stimulation by means of quantitative real-time polymerase chain reaction assays. A dose- dependent response of IGF-I mRNA was observed after pjGHr administration, and reached a six-fold IGF-I maximum increase over control group when 2.5 mg pjGH/g-body weight (bw) was injected. Temporal analysis of hepatic IGF-I mRNA level showed that administration of a single dose of pjGHr into juvenile pejerrey resulted in a significant increase (P<0.02) 9 hours post-injection (hpi). These results add novel information on the nucleotide sequence of IGF-I in Atheriniformes and demonstrate that pjGHr could promote a dramatic response in liver, increasing the IGF-I mRNA level.