IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Analysis of mechanisms of bacterial (Serratia marcescens) attachment, migration and killing of fungal hyphae
Autor/es:
MAYA, TAL; SHADKCHAN; YANA; FICHTMAN, BORIS; BRUNA, R.; RON, SAPIR; KIJNER, NITZAN; HAREL, AMNON ; GARCÍA VÉSCOVI, ELEONORA; HOVER, TAL; SANDOVSKY, HANI; MITIAGIN, YULIA; SHANKS, ROBERT; OSHEROV, NIR
Revista:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Lugar: Washington; Año: 2016 vol. 82 p. 2585 - 2594
ISSN:
0099-2240
Resumen:
We have found a remarkable capacity of the ubiquitous gram negative rod bacterium 33Serratia marcescens to migrate along and kill the mycelium of zygomycete molds. 34This migration was restricted to zygomycete molds, and several Basidiomycete spp. 35No migration was seen on any mold of the phylum Ascomycota. S. marcescens 36migration did not require fungal viability or surrounding growth medium, as bacteria 37migrated along aerial hyphae as well. S. marcescens did not exhibit growth tropism 38towards zygomycete mycelium. Bacterial migration along hyphae proceeded only 39when the hyphae grew into the bacterial colony. S. marcescens cells initially migrated 40along the hyphae, forming attached microcolonies that grew and coalesced to generate 41a biofilm covering and killing the mycelium. Flagellum-defective strains of S. 42marcescens were able to migrate along zygomycete hyphae, although significantly 43slower than the wild-type strain and were delayed in fungal killing. Bacterial 44attachment to the mycelium does not necessitate type 1 fimbrial adhesion since 45mutants defective in this adhesin migrated equally well or faster than the wild- type 46strain. Killing does not depend on the secretion of S. marcescens chitinases as mutants 47in which all three chitinase genes were deleted retained wild-type killing abilities. 48Better understanding of the mechanisms by which S. marcescens binds, spreads and 49kills fungal hyphae could serve as an excellent model system for such interactions in 50general; fungal killing could be employed in agricultural fungal biocontrol.