IBR 13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Annexin A2 is involved in pig (Sus scrofa) sperm-oviduct interaction.
Autor/es:
JUAN M. TEIJEIRO, GEORGE G. IGNOTZ, AND PATRICIA E. MARINI
Revista:
MOLECULAR REPRODUCTION AND DEVELOPMENT
Editorial:
Wiley-Liss, Inc
Referencias:
Año: 2009 vol. 76 p. 334 - 341
ISSN:
1040-452X
Resumen:
The oviduct is a dynamic organ which modulates gamete physiology.
Sperm-oviduct interaction provides the formation of a sperm storage
reservoir and allows the selection of sperm with certain qualities in
eutherian mammals. In sows, the oviductal sperm binding glycoprotein
(SBG) has been proposed to be involved in sperm selection. In this
work, based on its affinity to sperm periacrosomal membrane proteins,
we isolate another pig oviductal cell protein that interacts with
sperm. Peptide identification by LC/MS-MS allowed the identification of
this protein as annexin A2. The presence of this annexin, as well as
annexin A1 and annexin A5 in sow oviductal cells was confirmed by
Western blot with specific antibodies. The three proteins were
localized in sow oviduct by immunohistochemistry, showing the presence
of annexin A2 at the apical surface of the oviductal epithelial cells.
Based on our data and the fact that annexins have been stated as
candidate receptors of bovine sperm for sperm reservoir formation, we
propose that this family of proteins is involved in sperm-oviduct
interaction, annexin A2 being the main sperm binding isoform in pig.
