Metabolic analyses of interspecific tomato recombinant inbred lines for fruit quality improvement

LÓPEZ M; ZANOR MI; PRATTA GR; STEGMAYER G; BOGGIO SB; CONTE M; BERMUDEZ L; COLUCCIO LESKOW C; RODRÍGUEZ GR; PICARDI LA; ZORZOLI R; FERNIE AR; MILONE D; ASIS R; VALLE EM; CARRARI F

METABOLOMICS

SPRINGER

Lugar: Berlin; Año: 2015 vol. 11 p. 1416 - 1431

1573-3882

Elucidating the determinants of tomato nutritionalvalue and fruit quality to introduce improved varietieson the international market represents a majorchallenge for crop biotechnology. Different strategies canbe undertaken to exploit the natural variability of Solanumto re-incorporate lost allelic diversity into commercial varieties.One of them is the characterization of selectedgermplasm for breeding programs. To achieve this goal, 18RILs (S. lycopersicum 9 S. pimpinellifolium) were comprehensivelyphenotyped for fruit polar metabolites andquality associated traits. Metabolites were quantified byGC?MS and 1H NMR. Integrative analyses by neuronalclustering and network construction revealed that fruitproperties are strongly associated with the metabolites aspartate,serine, glutamate and 2-oxoglutarate. Shelf life andfirmness appeared to be linked to malate content. By acomparative analysis of the whole data set, ten RILs presentedhigher number of traits with positive effect than theS. lycopersicum 9 S. pimpinellifolium hybrid. Thus, theselines can be proposed as promising candidates for breedingprograms aimed to improve fruit quality.