Isolation and characterization of 20 polymorphic microsatellite loci in the migratory freshwater fish Leporinus obtusidens (Characiformes= Anostomidae) using 454 shotgun pyrosequencing

VILLANOVA GABRIELA VANINA; VERA MANUEL; DIAZ JUAN; MARTINEZ PAULINO; CALCATERRA NORA BEATRIZ; ARRANZ SILVIA EDA

JOURNAL OF FISH BIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2015

0022-1112

Twenty polymorphic microsatellite markers were developed for the Neotropical fish Leporinus obtusidens using a next generation sequencing approach and tested in two other characifomes species, Schizodon platae and Prochilodus lineatus. Microsatellite loci alleles in L. obtusidens ranged between 2 and 20 alleles per locus (mean=5⋅7), with expected heterozygosity values ranging from 0⋅097 to 0⋅956 (mean=0⋅578) and observed heterozygosity values ranging from 0⋅000 to 0⋅800 (mean=0⋅400) in a sample of 20 specimens from the lower Paraná River (Argentina). Most of these markers will be a valuable tool for captive breeding and stocking programmes, as well as for analyses of population connectivity and genetic structure in this broadly distributed Neotropical migratory fish.