IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Human initiation factor eIF3 subunit b interacts with HCV IRES RNA through its N-terminal RNA recognition motif.
Autor/es:
PÉRARD J; RASIA R; MEDENBACH J; AYALA I; BOISBOUVIER J; DROUET E; BAUDIN F
Revista:
FEBS LETTERS
Referencias:
Año: 2009 vol. 583 p. 70 - 74
ISSN:
0014-5793
Resumen:
Many viral mRNAs contain a 5′-UTR RNA element called internal
ribosome-entry site (IRES), which bypasses the requirement of some
canonical initiation factors allowing cap-independent translation. The
IRES of hepatitis-C virus drives translation by directly recruiting 40S
ribosomal subunits and binds to eIF3 which plays a critical role in
both cap-dependent and cap-independent translation. However, the
molecular basis for eIF3 activity in either case remains enigmatic.
Here we report that subunit b of the eIF3 complex directly binds to HCV
IRES domain III via its N-terminal-RRM. Because eIF3b was previously
shown to be involved in eIF3j binding, biological implications are
discussed.