IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
The Met Axial Ligand determines the redox potential in CuA Sites
Autor/es:
GABRIELA N. LEDESMA; DANIEL H. MURGIDA; HOANG KHOA LY; HAINER WACKERBARTH; JENS ULSTRUP; ANTONIO J. COSTA-FILHO; ALEJANDRO J. VILA
Revista:
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Editorial:
American Chemical Society
Referencias:
Año: 2007 vol. 129 p. 11884 - 11885
ISSN:
0002-7863
Resumen:
The replacement of the axial methionine ligand in a native Cu-A protein rendered a series of stable mutants with spectroscopic features of a mixed valence center. The mutations resulted in minor perturbations of the electronic structure of this site but led to significant changes in the redox potential. This suggests that the axial ligand plays a role in tuning the redox potential of Cu-A, resembling the behavior observed in blue copper sites.