IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Glycoproteins of the Vitelline Envelope of Amphibian Oocyte: Biological and Molecular Characterization of ZPC component (gp41) in Bufo arenarum
Autor/es:
BARISONE, G.A., KRAPF, D., CORREA-FIZ, F, ARRANZ, S.E. AND CABADA,
Revista:
MOLECULAR REPRODUCTION AND DEVELOPMENT
Referencias:
Año: 2007 vol. 74 p. 629 - 640
ISSN:
1040-452X
Resumen:
The vitelline envelope (VE) participatesin sperm-egg interactions during the first stepsof fertilization. In Bufo arenarum, this envelope iscomposed of at least four glycoproteins, with molecularmasses of 120, 75, 41, and 38 kDa and molar ratio of1:1.3:7.4:4.8, respectively. These components wereisolated and covalently coupled to silanized glass slidesin order to study their sperm-binding capacity. Whenconsidering the molar ratio of the glycoproteins inthe egg-envelope and assuming that each protein ismonovalent for sperm, the assay showed that gp41and gp38 possess 55 and 25% of total sperm-bindingactivity. We obtained a full-length cDNA of gp41(ZPC), comprising a sequence for 486 amino acids,with 43.3% homology with Xenopus laevis ZPC. As inthe case of mammalian ZP3 and Xenopus ZPC, BufoZPC presented a furin-like (convertase) and a Cterminaltransmembrane domain (TMD) reflectingcommon biosynthetic and secretory pathways. As itwas reported for some fishes, we obtained evidencethat suggests the presence of more than one zpc genein Bufo genome, based on different partial cDNAsequences of zpc, Southern blots and two-dimensionalSDS–PAGE of deglycosylated egg-envelope components.As far as we are aware, this is the firstobservation of the presence of different zpc genes inan Amphibian species.