Mismatch recognition function of Arabidopsis thaliana MutS-gamma

GÓMEZ, R.; SPAMPINATO, C.

DNA REPAIR

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2013 vol. 12 p. 257 - 264

1568-7864

Genetic stability depends in part on an efficient DNA lesion recognition and correction by the DNA mismatch repair (MMR) system. In eukaryotes, MMR is initiated by the binding of heterodimeric MutS homologue (MSH) complexes, MSH2/MSH6 and MSH2/MSH3, which recognize and bind mismatches and unpaired nucleotides. Plants encode another mismatch recognition protein, named MSH7. MSH7 forms a heterodimer with MSH2 and the protein complex is designated MutS-gamma. We here report the effect the expression of Arabidopsis MSH2 and MSH7 alone or in combination exert on the genomic stability of Saccharomyces cerevisiae. AtMSH2 and AtMutS-gamma proteins failed to complement the hypermutator phenotype of an msh2 deficient strain. However, overexpressing AtMutS-gamma in MMR proficient strains generated a 4-fold increase in CAN1 forward mutation rate, when compared to wild-type strains. CanR mutation spectrum analysis of AtMutS-gamma overproducing strains revealed a substantial increase in the frequency of base substitution mutations, including an increased accumulation of base pair changes from G:C to A:T and T:A to C:G, G:C or A:T. Taken together, these results suggest that AtMutS-gamma affects yeast genomic stability by recognizing specific mismatches and preventing correction by yeast MutS-alpha and MutS-beta, with subsequent inability to interact with yeast downstream proteins needed to complete MMR.