IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Set-up of an infrared fast behavioral assay using zebrafish (Danio rerio) larvae, and its application for high-throughput compound biotoxicity screening.
Autor/es:
BICHARA, D.; CALCATERRA, N.B.; ARRANZ, S.E.; ARMAS, P.; SIMONETTA, S.
Revista:
JOURNAL OF APPLIED TOXICOLOGY
Editorial:
JOHN WILEY & SONS LTD
Referencias:
Lugar: Londres; Año: 2014 vol. 34 p. 214 - 219
ISSN:
0260-437X
Resumen:
Zebrafish (Danio rerio) is increasingly employed for evaluating toxicity and drug discovery assays. Commonlyexperimental approaches for biotoxicity assessment are based on visual inspection or video recording. However, these techniquesare limited for large-scale assays, as they demand either a time-consuming detailed inspection of the animals or intensivecomputing resources in order to analyze a considerable amount of screenshots. Recently, we have developed a simplemethodology for tracking the locomotor activity of small animals cultured in microtiter plates. In this work, we implementedthis automatic methodology, based on infrared (IR) microbeam scattering, for measuring behavioral activity in zebrafishlarvae. We determined the appropriate culture conditions, number of animals and stage of development to get robust results.Furthermore, we validated this methodology as a rapid test for evaluating toxicity. By measuring the effects of referencecompounds on larvae activity, we were able to estimate the concentration that could cause a 50% decrease in activity eventsvalues (AEC50), showing a strong linear correlation (R2 = 0.91) with the LC50 values obtained with the standard DarT test. Thetoxicity order of the measured compounds was CuSO4>2,4-dinitrophenol>3,4-dichloroaniline>SDS>sodium benzoate>EDTA>K2CrO4; regarding solvents, EtOHDMSO. In this study, we demonstrate that global swimming behavior could bea simple readout for toxicity, easy to scale-up in automated experiments. This approach is potentially applicable for fastecotoxicity assays and whole-organism high-throughput compound screening, reducing the time and money required toevaluate unknown samples and to identify leading pharmaceutical compounds.