IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Peptide nanofibrils boost retroviral gene transfer and provide a rapid means for concentrating viruses.
Autor/es:
MARAL YOLAMANOVA; CHRISTOPH MEIER; ALEXEY K. SHAYTAN; VIRAG VAS; CARLOS W. BERTONCINI; FRANZISKA ARNOLD; ONOFRIO ZIRAFI; SHARIQ M. USMANI; JANIS A. MÜLLER; DANIEL SAUTER; CHRISTINE GOFFINET; DAVID PALESCH; PAUL WALTHER; NADIA R. ROAN; HARTMUT GEIGER; OLEG LUNOV; THOMAS SIMMET; JENS BOHNE; HUBERT SCHREZENMEIER; KLAUS SCHWARZ; LUDGER STÄNDKER; WOLF-GEORG FORSSMANN; XAVIER SALVATELLA; PAVEL G. KHALATUR; ALEXEI R. KHOKHLOV; TUOMAS P. J. KNOWLES; TANJA WEIL; FRANK KIRCHHOFF ; JAN MÜNCH
Revista:
NATURE NANOTECHNOLOGY
Editorial:
NATURE PUBLISHING GROUP
Referencias:
Lugar: Londres; Año: 2013 vol. 8 p. 130 - 136
ISSN:
1748-3387
Resumen:
Inefficient gene transfer and low virion concentrations are common limitations of retroviral transduction1.
We and others have previously shown that peptides derived from human
semen form amyloid fibrils that boost retroviral gene delivery by
promoting virion attachment to the target cells2, 3, 4, 5, 6, 7, 8.
However, application of these natural fibril-forming peptides is
limited by moderate efficiencies, the high costs of peptide synthesis,
and variability in fibril size and formation kinetics. Here, we report
the development of nanofibrils that self-assemble in aqueous solution
from a 12-residue peptide, termed enhancing factor C (EF-C). These
artificial nanofibrils enhance retroviral gene transfer substantially
more efficiently than semen-derived fibrils or other transduction
enhancers. Moreover, EF-C nanofibrils allow the concentration of
retroviral vectors by conventional low-speed centrifugation, and are
safe and effective, as assessed in an ex vivo gene transfer
study. Our results show that EF-C fibrils comprise a highly versatile,
convenient and broadly applicable nanomaterial that holds the potential
to significantly facilitate retroviral gene transfer in basic research
and clinical applications.