IBR 13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Molecular identification of the sperm selection involved porcine sperm binding glycoprotein (SBG) as deleted in malignant brain tumors 1 (DMBT1)
Autor/es:
TEIJEIRO JUAN M; ROLDÁN, LORENA; MARINI PATRICIA E
Revista:
BIOCHIMIE
Editorial:
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
Referencias:
Año: 2012 p. 263 - 267
ISSN:
0300-9084
Resumen:
Porcine sperm binding glycoprotein (SBG) is involved in sperm-oviduct interaction.Here we use mass spectrometry to identify SBG, finding peptides corresponding todeleted in malignant brain tumors 1 (DMBT1), at scavenger receptor cysteine-rich(SRCR) and CUB domains. RT-PCR allowed the cloning of unique sequences,belonging to porcine DMBT1. Western blot and immunofluorescence of oviductaltissues using anti-SBG and anti-hDMBT1 antibodies showed identical results. Thebiochemical characteristics of both proteins are coincident. We conclude that porcineSBG is an oviductal form of DMBT1, and thus assign this protein a novel location andfunction.*Abstract
