IBR   13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
Genetic diversity of Cercospora kikuchii isolates from soybean cultured in Argentina as revealed by molecular markers and cercosporin production
Autor/es:
LURÁ MC, LATORRE RAPELA MG, VACCARI MC, MAUMARY R, SOLDANO A, MATTIO M AND GONZÁLEZ AM
Revista:
MYCOPATHOLOGIA
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2011 vol. 171 p. 361 - 371
ISSN:
0301-486X
Resumen:
Leaf blight and purple seed, caused by the fungal pathogen Cercospora kikuchii (Matsumoto &Tomoyasu) M. W. Gardner are very important diseases of soybean (Glycine max L. Merr.) inArgentina. The aims of this work were: (a) to confirm and to assess the genetic variability amongC. kikuchii isolates collected from different soybean growing areas in Santa Fe province using inter simple sequence repeats (ISSR) markers and sequence information from the internal transcribed spacer (ITS) region of rDNA and (b) to analyze the cercosporin production of the regional C. kikuchi isolates in order to assess whether there was any relationship between the molecular profiles and the toxin production. Isolates from different regions in Santa Fe province were studied. The sequence of the ITS regions showed high similarity (99?100%) to the GenBank sequences of C. kikuchii BRCK179 (accession number AY633838). The ISSR markers clustered all the isolates into many groups and cercosporin content was highly variable among isolates. No relationship was observed between ITS region, ISSR groups and origin or cercosporincontent. The high degree of genetic variability and cercosporin production among isolates compared in this study characterizes a diverse population of C. kikuchii in the region.