Induction of Ammonium Metabolizing Enzymes is Related with Fruit Shelf Life in Tomato Crosses among Cultivated, Mutant and Exotic Germplasms

PRATTA GR, ZORZOLI R, PICARDI LA, VALLE EM

INTERNATIONAL JOURNAL OF PLANT BREEDING

Global Science Books

Año: 2007 vol. 1 p. 70 - 73

1752-3478

In a previous report, glutamine synthetase (GS) and glutamate dehydrogenase (GDH) were found to be differentially induced in fruits at two ripening stages among tomato lines with different shelf life (SL). The objective of the present research was to analyze SL and the induction of GS and GDH in the pericarp tissue at the mature-green and the red ripe stages in different tomato genotypes, including a set of hybrids and their parents. The L. esculentum parents were the standard ripening cultivar ‘Caimanta’, which produces round freshmarketable fruits, and two inbred lines, N and R, which are recessive for the nor and rin mutations, respectively. The exotic parent ‘Ceras’ belonged to the wild L. esculentum var. cerasiforme. Four crosses were assayed: F1 (‘Caimanta’ x N), F1 (‘Caimanta’ x ‘Ceras’), F1 (N x ‘Ceras’), and F1 (N x R). The concomitant presence of GS and GDH was found in the pericarp of mature-green and red ripe tomato fruits from N, R, ‘Ceras’, F1 (‘Caimanta’ x ‘Ceras’), F1 (N x ‘Ceras’), and F1 (N x R). All these genotypes had a long SL. Particularly, in red ripe fruits of N and R, the genotypes with the longest SL, two isoforms of GDH were detected. The F1 (‘Caimanta’ x N) had a quite shorter SL and presented a differential induction pattern: while GS was found just in mature-green fruits, GDH was present at both ripening stages.In ‘Caimanta’, the genotype with shortest SL, GS was present only at the mature-green stage and GDH at the red ripe stage.