IBR 13079
INSTITUTO DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Unidad Ejecutora - UE
artículos
Título:
ISAba825, a functional insertion sequence modulating genomic plasticity and bla(OXA-58) expression in Acinetobacter baumannii
Autor/es:
RAVASI, P.; LIMANSKY, A.S.; RODRIGUEZ R.; VIALE A.M.; MUSSI M.A.
Revista:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Año: 2011 vol. 55 p. 917 - 920
ISSN:
0066-4804
Resumen:
ISAba825, an insertion sequence found inactivating Acinetobacter baumannii carO, was tagged with a kanamycin (Kn) resistance cassette. ISAba825::Kn effectively transposed in A. baumannii, showing preference for short, AT-enriched target sequences, generating 6- to 9-bp target duplications. Additionally, we detected the presence of ISAba825 upstream of a plasmid-borne bla(OXA-58) gene, generating a hybrid promoter largely enhancing its expression and leading to carbapenem resistance. Overall, a role for ISAba825 in carbapenem resistance modulation in A. baumannii is proposed.
