Emergence of Pseudomonas aeruginosa strains producing metallo- beta-lactamases of the IMP-15 and VIM-2 types in Mexico.

QUINONES-FALCONI, F.; GALICIA-VELASCO, M.; MARCHIARO, P.; MUSSI M.A.; BALLERINI, V.; VILA, A.J.; VIALE, A.M.; BERMEJO-MORALES, K.; LIMANSKY, A.S.

CLINICAL MICROBIOLOGY AND INFECTION

European Society of Clinical Microbiology and Infectious Diseases

Año: 2010 vol. 16 p. 126 - 131

1198-743X

Eighty-six carbapenem non-susceptible Pseudomonas aeruginosa isolates collected in the National Institute of Respiratory Diseases of Mexico City were screened for the presence of metallo-beta-lactamase (MBL) activity using both E-test strips and a microbiological assay with EDTA-imipenem. Genomic comparisons and sequence analyses conducted with these isolates revealed the presence of bla(VIM-2) in two clonally related isolates, and bla(IMP-15) in a clonally unrelated isolate. Both genes were found to be carried by class 1 integrons, and bla(IMP-15) was additionally present on a broad host-range plasmid. This is the first report of co-existing P. aeruginosa strains producing different MBLs in a Mexican hospital, highlighting the necessity of appropriate surveillance to prevent dissemination of carbapenem resistance.