Peripheral nerve regeneration promoted by adipose-derived stem cell (AdSC) magneto targeting

G. PIÑERO; V. USACH; P. SETTON-AVRUJ; P. A. SOTO; M. B. FERNÁNDEZ VAN RAAP

Cordoba

Congreso; XXXIII Annual meeting of the Argentine Society for Research in Neuroscience; 2018

SAN Sociedad Argentina de Investigacion en Neuro Ciencia

Wallerian degeneration (WD) is an efficient animal experimental model in mimicking the impact of peripheral nerve lesion to shed light on possible regeneration strategies. AdSC transplant is a useful tool for regenerative therapies, while magneto targeting is a nanotechnological strategy to mobilize magnetic nanoparticle (MNP)-loaded cells to a specific tissue guided by an external magnetic field. In this context, the aim of the present work was to test whether AdSC-MNP magneto targeting can enhance the regenerative ability of AdSC upon rat sciatic nerve lesion.To this end, cultured AdSC were characterized for multipotent cell marker expression. MNP internalization was evaluated through transmission electron microscopy and vibrating sample magnetometry (VSM) experiments. Likewise, epifluorescence microscopy and VSM analyses were performed to evaluate the arrival of AdSC-MNP at the injured nerve. Finally, AdSC-MNP transplantation effects on nerve morphology and conduction were evaluated through immunofluorescence, Western blot and electrophysiological experiments.Our results show that AdSC express CD105, CD90 and CD34, and can internalize 2 to 4 pg MNP/cell. We demonstrate AdSC-MNP to supersede AdSC arrival exclusively at the lesion site, exerting beneficial effects on nerve morphology and conduction.In short, our results prove that AdSC-MNP magneto targeting constitutes a valuable tool to enhance AdSC arrival at the lesion site and consequent nerve regeneration.