INSTITUTO DE FISICA LA PLATA
Unidad Ejecutora - UE
congresos y reuniones científicas
Synthesis and cell internalization of magnetite nanoparticles for magnetic hyperthermia applications
M. E. DE SOUSA; P. GIRARDIN; M. B. FERNÁNDEZ VAN RAAP; P. MENDOZA ZÉLIS; G. PASQUEVICH; I. J. BRUVERA; F. H. SÁNCHEZ; R. G. GOYA; Y. E. SOSA
Conferencia; HK 2010 - Humboldt Kolleg; 2011
Physics Department - Faculty of Exact Sciences National University of La Plata
Magnetic hyperthermia treatment appears as a viable alternative or complementary procedure for localized cancerous tumors therapy. The treatment consists of locally injecting magnetic nanoparticles (MNP) into the tumor and exposing it to an oscillating magnetic field in the radio frequency range. When exposed to this field, the MNP dissipates energy causing a temperature increase in the zone that contains nanoparticles, inducing tumor cell death by apoptosis at or above 42 °C. MNP energy dissipation is characterized by the specific power loss parameter (SLP) which expresses the power absorbed by the MNP from the field per mass unit of MNP. 13 nm size magnetite nanoparticles with saturation magnetization of 60 emu/g, were synthesized by co-precipitation route using iron salts and a strong base, and finally set into aqueous suspension. Their physical properties were characterized with various techniques and suggest their adequacy for hyperthermia treatment and cell internalization requirements. Measured SPL value for this solution, using a coil fed with a signal of 260 kHz and amplitude of 500 Oe (39.8 kA m-1), was 203 W g-1. Internalization methodology for anionic magnetic nanoparticles, which is achieved, for instance, by adsorption of citrate anions to the ferric oxide surface, is known to be well established in various tumoural and normal cells. Here, bare MNP nanoparticle were internalizated in A549 cells from human lung adenocarcinoma for two NP concentrations of 20 and 50 µg/ml. MNP incorporation into cells follows the endocytic pathway. It is observed that MNP concentration inside cells is larger and more densely packed for the larger MNP concentration. Confocal, atomic force and transmission electron microscopies were used to image the in vitro uptake of nanoparticles by A549 cells.