IFLP   13074
INSTITUTO DE FISICA LA PLATA
Unidad Ejecutora - UE
artículos
Título:
Fluorescent and magnetic stellate mesoporous silica for bimodal imaging and magnetic hyperthermia
Autor/es:
TASSO, MARIANA; BLANCO-ANDUJAR, CRISTINA; BÉGIN, DOMINIQUE; MERTZ, DAMIEN; MUÑOZ MEDINA, GUILLERMO A.; PORTIANSKY, ENRIQUE; MEYER, FLORENT; PERTON, FRANCIS; MÉNARD, MATHILDE; VAN RAAP, MARCELA B. FERNÁNDEZ; BEGIN-COLIN, SYLVIE
Revista:
Applied Materials Today
Editorial:
Elsevier
Referencias:
Año: 2019 vol. 16 p. 301 - 314
ISSN:
2352-9407
Resumen:
There is currently a crucial need of innovative multifunctional nanoparticles combining, in one formu-lation, imaging and therapy capacities allowing thus an accurate diagnosis and a therapy monitored byimaging. Multimodal imaging will ensure to speed up diagnosis, and to increase its sensitivity, relia-bility and specificity for a better management of the disease. Combined with a therapeutic action, itwill also enable to treat the disease in a specific personalized manner in feedback mode. The mastereddesign of such bioprobes as well as the demonstration of their efficiency are still challenges to facein nanomedicine. In this work, novel fluorescent and magnetic core?shell nanocomposites have beendesigned to ensure, in one nanoformulation, bimodal fluorescence and MRI imaging coupled with ther-apy by magnetic hyperthermia. They consist in the coating of a magnetic iron oxide (IO) core (ca. 18 nmdiameter to ensure magnetic hyperthermia) by an original large pore stellate mesoporous silica (STMS)shell to produce uniform and mono-core magnetic core?shell nanocomposites denoted IO@STMS NPs. Toconfer fluorescence properties, CdSe/ZnS quantum dots (QDs) NPs were grafted inside the large pores ofthe IO@STMS nanocomposites. To provide biocompatibility and opsonization-resistance, a tightly-boundhuman serum albumin (HSA) coating is added around the nanocomposite using an original IBAM-basedstrategy. Cellular toxicity and non-specific cell?nanomaterial interactions allowed to determine a con-centration range for safe application of these NPs. Cellular endosomes containing spontaneously-uptakenNPs displayed strong and photostable QD fluorescence signals while magnetic relaxivity measurementsconfirm their suitability as contrast agent for MRI. HeLa cell-uptaken NPs exposed to a magnetic fieldof 100 kHz and 357 Gauss (or 28.5 kA m−1) display an outstanding 65% cell death at a very low iron con-centration (1.25 g Fe mL−1), challenging current magnetic hyperthermia nanosystems. Furthermore, atthe particularly demanding conditions of clinical use with low frequency and amplitude field (100 kHz,117 Gauss or 9.3 kA m−1), magnetic hyperthermia combined with the delivery of a chemotherapeuticdrug, doxorubicin, allowed 46% cell death, which neither the drug nor the NPs alone yielded, evidencingthus the synergistic effect of this combined treatment.