REGULATION BY MIRNAS OF THE STABILITY OF RAC3 DURING ADIPOGENESIS

MATIAS EZEQUIEL BALDI; MILENI SOARES MACHADO; MONICA ALEJANDRA COSTAS; FRANCISCO DAMIÁN ROSA; GABRIELA INES MARINO; MARIA CECILIA LIRA; ALEJANDRA G. PALMA; MARIA FERNANDA RUBIO

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Congreso; LXV Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2020

Sociedad Argentina de Investigación Clínica

Adipogenesis is regulated by several adipocyte-selective microRNAs (miRNAs) and transcription factors. We have previously demonstrated that the expression of the transcriptional coactivatorRAC3 decreases during adipocyte differentiation, favoring the process through an increase of autophagy and a decrease in the proliferative rate.In order to determine the mechanism whereby RAC3 expression levels are regulated during adipogenesis, we performed public datasets analysis and evaluated RAC3 and miRNAs expressionduring adipocyte differentiation (GSE20696 and 20298). After 7 days of differentiation, in the murine preadipocyte 3T3-L1 cell line (GSE20696), we observed RAC3 expression was downregulated(43.6% +/- 0.9% respect to control, p1.5-fold change respect to control).We could conclude that the regulation of RAC3 expression during adipocyte differentiation not only occurs at transcriptional level but also may involve post-transcriptional regulatory mechanisms th