Cystic fibrosis transmembrane regulator (CFTR) in human trophoblast HTR-8/SVneo cells modulates several processes of the placental behavior

GABRIELA MARINO; BASILIO A. KOTSIAS; MARÍA FERNANDA RUBIO; ALEJANDRA G. PALMA; MÓNICA A. COSTAS

Buenos Aires

Congreso; Reunión Conjunta de Sociedades Biomédicas; 2017

SAIC

The expression of different channels and transporters is altered inpreeclamptic placentas, including the cystic fibrosis regulatory channel(CFTR), and defective placentation is one of the main features ofthis gestational disorder.Our aim was to study the expression and participation of placentalCFTR in the proliferation, migration, invasion and apoptosis duringplacentation.We observed by Western blot in first trimester extravillous trophoblastcell line (HTR8/SVneo) the typical band of ~160 KDa of CFTR.The silencing of the CFTR with a specific siRNA (50nM) showed ahalf decrease channel expression compared to control without treatmentand the scramble at 48-72h (p