Expression of pannexin1 in Xenopus Laevis oocyte

LOSAVIO ADRIANA ; GALIZIA LUCIANO; ALLEVA KARINA; SWATZBAUM PABLO JULIO; JUARRACINO, JUAN

Buenos Aires

Congreso; Congreso conjunto de Sociedades de Biociencias; 2017

Six subunits of Pannexin 1 (Panx1) form a plasma membrane channel termed ?pannexon?. Depending on the mode of stimulation, the pannexon has large conductance and unselective permeability to molecules less than 1.5 kD or is a small, chloride-selective channel. Mechanical stimuli or the increase of extracellular potassium concentration (K+)e induce the large channel conformation whereas the small conductance conformation so far has only been observed with exclusive voltage dependency of the channel. The properties of Panx1 channels in the large conductance conformation indicate that this protein is the most probable candidate for an ATP release. Although mechanical stimuli has been demonstrated to elicit ATP release trough Panx1, the sensitivity of pannexons to osmotic stimuli remains controversial. Expression of Panx1 in Xenopus oocytes was assessed by voltage clamp technique. Two to five days after mPanx1 cRNA injection, voltage dependent outward currents where observed. This currents were absent in water injected oocytes (Iss 60 mV = 0.60 ± 0.12 μA, n=10 vs 0.12 ± 0.01 μA, n=7, p