Increased megakaryopoiesis and thrombopoiesis induced by plasma from Myelofibrosis (MF) and Essential Thrombocythemia (ET) patients

MARTA RF; DISCIANNI LUPI AD; LEV PR; YAÑUK DANILA; DE LUCA G; GOETTE NP; CELLUCCI AS; HELLER PG

Mar del Plata

Congreso; LXVII Reunión anual de la sociedad argentina de investigación clínica; 2022

SAIC - SAI - SAFIS

MF and ET are characterized by abnormal proliferation of hematopoietic progenitors and chronic inflammation. MF presents medullary fibrosis, which confers a worse prognosis. Our objective was to evaluate the effect of plasma from MF and ET patients on megakaryo/thrombopoiesis.Megakaryopoiesis was evaluated in CD34+ hematopoietic progenitors (umbilical cord blood) cultured with TPO and 10% recalcified plasma (patients n=51, 29 MF and 22 ET; controls n=45) for 12 days. Total cell number (proliferation) and percentage of CD61 (differentiation) and CD42b (maturation) cells were quantified by optical microscopic and by flow cytometry, respectively. Thrombopoiesis was evaluated in mature megakaryocytes (MKs) cultured for 72 hours with TPO and 10% recalcified plasma. Proplatelets (PP) were counted using an inverted microscope and PP morphology was analyzed by immunofluorescence. Statistical analysis was performed using Mann Whitney and Spearman´s correlation tests.An increase in MKs number and maturation was observed in the presence of plasma from MF compared to ET and control (p