IDIM 12530
INSTITUTO DE INVESTIGACIONES MEDICAS
Unidad Ejecutora - UE
artículos
Título:
Phytomonas: a non-pathogenic trypanosomatid model for functional expression of proteins
Autor/es:
MIRANDA MR; SAYE M; REIGADA C; CARRILLO C; PEREIRA CA
Revista:
PROTEIN EXPRESSION AND PURIFICATION
Editorial:
ACADEMIC PRESS INC ELSEVIER SCIENCE
Referencias:
Lugar: Amsterdam; Año: 2015
ISSN:
1046-5928
Resumen:
Phytomonas are protozoan parasites from the Trypanosomatidae family whichinfect a wide variety of plants. Herein, Phytomonas Jma was tested as a modelfor functional expression of heterologous proteins. Green fluorescent proteinexpression was evaluated in Phytomonas and compared with Trypanosomacruzi, the etiological agent of Chagas? disease. Phytomonas was able toexpress GFP at levels similar to Trypanosoma cruzi although the transgenicselection time was higher. It was possible to establish an efficient transfectionand selection protocol for protein expression. These results demonstrate thatPhytomonas can be a good model for functional expression of proteins fromother trypanosomatids, presenting the advantage of being completely safe forhumans.
