CHANGES IN HULL CELL WALLS STRUCTURE PRODUCED BY ATTACK OF STINKBUG NEZARA VIRIDULA ON DEVELOPING SOYBEAN SEEDS

FRANCO ARIAS ILABACA; MARINA CIANCIA; JORGE A. ZAVALA

cordoba

Congreso; XXXII Reunión Argentina de Fisiología Vegetal (RAFV) y XVI Congreso Latinoamericano de Fisiología Vegetal; 2018

Sociedad Argentina de Fisiología Vegetal

Nezara viridulais one the most important pests in soybean crops. Soybean responds to stinkbugattack inducing defenses regulated by salicylic acid. Cell walls can be aneffective barrier against piercing-sucking insects. In mature soybeancarbohydrates make up 35% of (SB) seeds. No detailed study on hullpolysaccharides in the immature stage, when Nezara attack takes place.The aim of this work was to determine if stinkbug damage produces changes incell wall polysaccharides.Hull cell wallpolysaccharides from seeds of a commercial cultivar were studied. One untreated(CTR) lot and two different treatments were prepared, during R5 stage: (1)stinkbug damaged ? insects were placed on pods and enclosed with a fine meshbag, during 15 days (BD); (2) pods sprayed with 1.5 mM of salicylic acid every4 days, during 15 days (TSA), This were collected in R6 stage. Sequentialextraction with water, 0.05 M CDTA pH 6, 0.05 M Na2CO3, 1M KOH, and 4M KOH wascarried out. Analyses showed major differences between treatments instructure of mannans, and non-esterified homogalacturonans (HG) and in theratio RGI:methyl-esterified HG. These results show that stinkbug attackproduces measurable changes in hull cell wall structure from Glicinemax.