Real-time reaction monitoring by probe electrospray ionization mass sepectrometry.

ZHAN YU; LEE CHUIN CHEN; HIROSHI NONAMI; KENZO HIRAOKA; ERRA BALSELLS, ROSA

RAPID COMMUNICATIONS IN MASS SPECTROMETRY : RCM.

JOHN WILEY & SONS LTD

Lugar: Londres; Año: 2010 vol. 24 p. 1507 - 1513

0951-4198

Abstract          Probe electrospray ionization (PESI) is a modified version of the conventional electrospray ionization (ESI), where the capillary is replaced by a solid needle. Capabilities of high tolerance for salts and direct ambient sampling are major advantages of PESI. In this study, PESI was interfaced with a time of flight mass spectrometer (TOF-MS) to monitor the real-time process of some biological and chemical reactions. By PESI-MS, time-resolved mass spectra and ion chromatograms can be easily obtained. In addition, real-time PESI-MS monitoring can also give qualitative and quantitative information of changes of each chemical species in detail, which are valuable for better understanding of the whole process of reactions and optimization of related parameters for a better control of the reactions. Therefore, PESI-MS can be considered as a new choice of tools for reactions monitoring in real-time because of its simplicity in instrumental configuration, direct sampling without sample preparation and low sample consumption.