Glucuronoarabinoxylans as major cell walls polymers from young shoots of the woody bamboo Phyllostachys aurea.

ZELAYA, V. M.; FERNÁNDEZ, P. V.; ANDREA S. VEGA; MANTESE, A. I.; FEDERICO, A. A; CIANCIA, M.; ZELAYA, V. M.; FERNÁNDEZ, P. V.; ANDREA S. VEGA; MANTESE, A. I.; FEDERICO, A. A; CIANCIA, M.

CARBOHYDRATE POLYMERS

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2017 vol. 167 p. 240 - 249

0144-8617

tYoung shoots of Phyllostachys aurea showed glucuronoarabinoxylans (GAX) as the major hemicellulosiccomponents, being extracted in major amounts with 1 M KOH (ratio Xyl:Ara:GlcA, 100:67:8), but alsowith water, showing a broad structural variability. Mixed linkage glucans were also present, but in minoramounts, mostly concentrated in the 4 M KOH extracts, while pectin polymers were very scarce. Arabino-galactan proteins were an important part of water extracts, determined by the presence of the typicalarabinogalactan structures (3- and 6-linked Gal p; terminal and 5-linked Ara f), in addition to smallamounts of hydroxyproline (2?3% of total protein) and positive reaction to Yariv?s reagent. Morphologi-cal and anatomical characteristics of young shoots are described, as well as localization of some cell wallcomponents, and related with chemical analysis. A method for determination of uronic acids as theirN-propylaldonamide acetates and separation and quantification by GC/MS was adapted for its use withgrass cell wall fractions.