Blossom End Rot Tomato Fruit Diagnosis for In Situ Cell Analyses with Real Time Pico-Pressure Probe Ionization Mass Spectrometry.

H. NONAMI; R. ERRA-BALSELLS; Y. GHOLIPOUR, ; ERRA BALSELLS, ROSA

Environmental Control in Biology

Yokendo

Lugar: Tokyo; Año: 2017 vol. 55 p. 41 - 51

1880-554X

By combining a cell pressure probe and an Orbitrap mass spectrometer, quantitative snapshot profiles of metabolites of in situ plant single cells during development of blossom end rot in tomato (Solanum lycopersicum L.) fruit were analyzed while tomato plants weregrown hydroponically in a greenhouse. By using the pressure probe, cell turgor, cellvolume, cell wall elastic modulus, and hydraulic conductivity of plasma membrane were measured, followed by managed cytoplasm sampling, and osmotic and water potentials determination. The picoliter cell sample was then directly ionized and injected under a high voltage condition into the mass spectrometer and its metabolite contents were profiled and quantified. It was found that the cell bursting results from alterations in water relations and cell wall properties accompanied with changes in metabolites of cells located at blossom end rot area in tomato fruit. From the water relations point of view, the loss of the elasticity and cell wall weakening, with decreased water potential and excess turgor resulted in mechanical rupture of membrane and cell wall. Abscisic acid was detected in damaged cells as a possible evidence of triggered precocious maturity.