Several electrochemical devices for biomolecular detection

GRACIELA A. GONZÁLEZ*, ANA SOL PEINETTI, SOFÍA CARUGNO, MAURICIO GÜNTHER, GRACIELA PRIANO, DIEGO PALLAROLA, AND FERNANDO BATTAGLINI

Playa del Carmen, Mexico

Conferencia; Electrochemistry Zing Conference; 2009

Zing Conference

Resumen de la Presentación Oral We report two examples of electrochemical systems aimed to sensor development. One is based on amperometric detection, using an inexpensive eight gold electrode array1. The array is manufactured by a combination of screen printing and gold electrodeposition techniques, used with a multipotenciostat, works as an efficient array to develop recognition assays with electrochemical detection. The detection of lipopolysaccharides and peroxidase, as examples of competitive or sandwich assays, will be presented. The other application compromises a nanoporous membrane2 placed close to the working electrode. In this case the signal is generated by a probe; therefore there is no need for analytes or labeled analytes able to produce an amperometric response. Two types of membranes were studied. The experimental results of the electrochemical system were modeled by numerical simulation. The effect of porous domains in the amperometric response of cyclic voltammetry experiments is simulated to establish the sensitivity of this system to detect molecules able to block the pores. The generated data is in good agreement with experimental results. The experimental sensitivity of the three systems can be observed through the current response of modified surface with anti-HRP-HRP complex.1. The array is manufactured by a combination of screen printing and gold electrodeposition techniques, used with a multipotenciostat, works as an efficient array to develop recognition assays with electrochemical detection. The detection of lipopolysaccharides and peroxidase, as examples of competitive or sandwich assays, will be presented. The other application compromises a nanoporous membrane2 placed close to the working electrode. In this case the signal is generated by a probe; therefore there is no need for analytes or labeled analytes able to produce an amperometric response. Two types of membranes were studied. The experimental results of the electrochemical system were modeled by numerical simulation. The effect of porous domains in the amperometric response of cyclic voltammetry experiments is simulated to establish the sensitivity of this system to detect molecules able to block the pores. The generated data is in good agreement with experimental results. The experimental sensitivity of the three systems can be observed through the current response of modified surface with anti-HRP-HRP complex.2 placed close to the working electrode. In this case the signal is generated by a probe; therefore there is no need for analytes or labeled analytes able to produce an amperometric response. Two types of membranes were studied. The experimental results of the electrochemical system were modeled by numerical simulation. The effect of porous domains in the amperometric response of cyclic voltammetry experiments is simulated to establish the sensitivity of this system to detect molecules able to block the pores. The generated data is in good agreement with experimental results. The experimental sensitivity of the three systems can be observed through the current response of modified surface with anti-HRP-HRP complex.