INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
An Alternative Method for the Detection of Endotoxins
BATTAGLINI FERNANDO; PRIANO GRACIELA; PALLAROLA DIEGO; GÜNTHER MAURICIO
NewPort, RI, USA
Congreso; Gordon Research Conference on Chemical Sensors and Interfacial Design; 2007
During the last twenty years, a great improvement has been made in the study of chemical interactions between lipopolysaccharides (LPS) and antibacterial agents produced by many organisms. These findings allow the development of an assay equivalent to those based upon antigen-antibody interaction, where the selective recognition is combined with a physicochemical change, which is used as a transducer element generating a signal proportional to the concentration of the analyte to be detected. In this work, we present different strategies for the detectecion of LPS using a competitive electrochemical assay, a competitive fluorescent assay and a label-free assay based on changes in capacitance followed by impedance spectroscopy. In all of them a recombinant endotoxin neutralizing protein (ENP) from Saccharomyces cerevisiae is used as recognition element. Sensors constructed according to the electrochemical configuration were able to detect the presence of endotoxins in concentrations of 0.2 EU/mL. The results obtained open new routes for a molecular recognition assay for endotoxins.