INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Endotoxin Detection Based In Molecular Recognition
Autor/es:
PALLAROLA DIEGO; PRIANO GRACIELA; GÜNTHER MAURICIO; GONZÁLEZ GRACIELA; BATTAGLINI FERNANDO
Lugar:
INQUIMAE, FCEyN, Ciudad Autónoma de Buenos Aires, Argentina.
Reunión:
Workshop; Second Max Planck Society sponsored German-Argentine Workshop, Multiparametric Probes and Agents of Cellular Function; 2007
Institución organizadora:
Max Planck Society
Resumen:
In this work, we propose a competitive assay for the detection of endotoxins with a recombinant Endotoxin Neutralizing Protein (ENP) as recognition element, avoiding lymph, blood, animals as part of the assay.  This simple assay opened some new strategic works: synthesis of LPS-PROBES (enzymes and fluorophores) conjugates, design and construction of disposable electrodes and studies of platforms for electrochemical affinity assays that avoid non-specific adsorption of biomolecules.
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