INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
Nitric oxide is reduced to HNO (azanone) by ascorbic acid, tyrosine, and other alcohols. A new route for HNO formation in biological media?
MARTINA MUÑOZ; SEBASTIÁN A. SUÁREZ; NICOLÁS NEUMAN; LUCIA ALVAREZ; MILJKOVIC J.; CARLOS BRONDINO; DAMIÁN E. BIKIEL; IVANOVIC-BURMAZOVIC I.; FILIPOVIC M. R.; MARCELO A. MARTÍ; FABIO DOCTOROVICH
Congreso; IV LABIC; 2014
The key role of nitric oxide (NO) in several biological processes seems nowadays to be well established. However, it should be considered that azanone (HNO, nitroxyl), the one electron reduction product of NO, could also be involved in some of these processes. The key pitfall for a full understanding of NO/HNO chemistry has been the lack of direct and specific methods to detect HNO. However, in the last few years several detection and trapping methods have been developed,(1?8) which opened the opportunity for detailed studies of its biological relevant reactions. In this context, one of the most important yet unanswered questions is whether HNO is produced in-vivo or not. One of the possible routes concerns chemical or enzymatic reduction of NO. In the present work we have taken advantage of a highly selective and sensitive HNO trapping and quantification method, to show that NO is reduced to HNO by several physiological moderate reductants, like ascorbic acid (AA), phenol (Ph-OH), hydroquinone (HQ) and tyrosine (Tyr). EPR evidence for the formation of the corresponding radicals has also been found.