INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
congresos y reuniones científicas
EFFECT OF NYTROXYL AND NITRIC OXIDE ON HUMAN PLATELET ARACHIDONATE METABOLISM
BERMEJO, EMILSE; SÁENZ, DANIEL; CHIANELLI. MONICA; BARI, SARA E.; LAZZARI, M.A.; ROSENSTEIN, RUTH
Congreso; XXIst Congress of the International Society on Thrombosis and Haemostasis; 2007
Nitric oxide (NO) plays a key role in platelet physiology regulation. Recent interest has developed in the function of an alternative redox form of NO, namely nitroxyl (HNO), because it is formed by diverse biochemical reactions. We have shown that both HNO and NO significantly inhibited human platelet aggregation induced by thrombin (Thr). The aim of the present work was to study the involvement of arachidonate metabolism in the antiaggregatory effect of HNO and NO. Methods: Human washed platelets (WP) from healthy volunteers were used throughout. Angeli´s salt (AS) and sodium nitroprusside (SNP) were used as HNO and NO releasers, respectively. Cyclic cGMP content was assessed by radioimmunoassay, while thiobarbituric acid reactive substances (TBARS) were measured as an index of lipid peroxidation. Thromboxane B2 (TXB2) levels were assessed by an immunoassay kit. Results: Both SNP and AS significantly increased cGMP content, although SNP was more effective than AS (control: 7.9±0.7, AS: 14.2±0.6, SNP: 32.2±3 pmol/10*6 WP). AS, SNP, and a cGMP analogue (8Br-cGMP) significantly decreased the effect of Thr on TBARS content. In addition, both releasers and 8-Br-cGMP significantly decreased the effect of Thr on TXB2 levels. Also in those cases, SNP was more effective than HNO. Both releasers and 8Br-cGMP showed no effect on these parameters in the absence of Thr.