INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
capítulos de libros
Título:
ADVANCED BIOMATERIALS: HYDROGELS THAT ALLOW DIVISION AND GROWTH OF ENCAPSULATED CELLS
Autor/es:
MERCEDES PERULLINI,; ANA CECILIA SPEDALIERI; MATIAS JOBBAGY; ALDABE BILMES, S
Libro:
Hydrogels: synthesis, characterization and applications
Editorial:
Nova Science Publishers, Inc.
Referencias:
Lugar: NY; Año: 2012; p. 1 - 19
Resumen:
Sol-gel encapsulation of living cells within inorganic matrices is an area of great scientific and technological interest. However, only recently a two-step procedure allowing population growth inside the hydrogel matrix has been proposed. In conventional one-pot encapsulations, space limitation suffered by confined cells makes their division completely impossible, and only intrinsic resistant microorganisms (mainly bacteria and yeasts) were encapsulated using extremely biocompatible synthesis procedures in order to obtain an acceptable viability in the resulting biomaterial. The two-step procedure based on a pre-encapsulation of the biological guest within Ca(II)-alginate, has proved to be a versatile method for advanced biomaterials development. For instance, in applications were it is necessary to keep a large number of metabolically active cells to render them efficient, or where the encapsulation of actively growing cells may be required. Moreover, it opened up many different possibilities to biomaterials, allowing the encapsulation of higher organisms cells (more sensitive than microorganism) as carrot phloematic tissue and tobacco BY2 Line or even of multicellular organisms, such as filamentous fungi. On the other hand, while one-pot encapsulations uses almost exclusively silica hydrogel hosts, the protection conferred to the cells by the pre-encapsulation step during the sol-gel synthesis procedure allows to extend this approach to other sol?gel materials, as has recently been addressed for Al(III) and Zr(IV) oxohydroxide hydrogels. This work constitutes the first observation of Escherichia coli growth within such metal oxide hydrogels, giving clear evidence that sol?gel based cell encapsulation can now be envisioned within a wide variety of metal oxide hosts through the optimization of the pre-encapsulation environment.