INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Re-absorption of chlorophyll fluorescence in leaves revisited. A comparison of correction models
Autor/es:
GABRIELA B. CORDON AND M. GABRIELA LAGORIO
Revista:
Photochemical and Photobiological Sciences
Editorial:
RSCPublishing
Referencias:
Lugar: Cambridge; Año: 2006 vol. 5 p. 735 - 740
ISSN:
1474-905X
Resumen:
The application of correction methods to account for re-absorption of chlorophyll .uorescence emission
in leaves is subject to a number of controversies in the literature. These uncertainties lead to high discrepancies
in the corrected spectral distribution of .uorescence and consequently in the interpretation of
related physiological features of plants, according to the chosen method used in the process of correction.
In this research, three correction methods, based on transmittance and/or re.ectance measurements on
leaves, were analysed comparatively. One method gave high values for the corrected .uorescence ratio
between 685 nm and 737 nm (F685/F737 7 to 20 according to the different species of leaves). The two
other methods were found to give similar results with corrected .uorescence ratios around a value of two
(F685/F737 2). While the .rst method was developed in the light of empirical considerations, the latter
two models are based upon de.ned physical approaches depicting interaction between light and matter.
The theoretical basis of these methods, the validation methodologies used to support them and the
similarity in the spectra corrected by light re-absorption for both models, all showed that they should be
treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.
two models are based upon de.ned physical approaches depicting interaction between light and matter.
The theoretical basis of these methods, the validation methodologies used to support them and the
similarity in the spectra corrected by light re-absorption for both models, all showed that they should be
treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.
other methods were found to give similar results with corrected .uorescence ratios around a value of two
(F685/F737 2). While the .rst method was developed in the light of empirical considerations, the latter
two models are based upon de.ned physical approaches depicting interaction between light and matter.
The theoretical basis of these methods, the validation methodologies used to support them and the
similarity in the spectra corrected by light re-absorption for both models, all showed that they should be
treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.
two models are based upon de.ned physical approaches depicting interaction between light and matter.
The theoretical basis of these methods, the validation methodologies used to support them and the
similarity in the spectra corrected by light re-absorption for both models, all showed that they should be
treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.
F685/F737 7 to 20 according to the different species of leaves). The two
other methods were found to give similar results with corrected .uorescence ratios around a value of two
(F685/F737 2). While the .rst method was developed in the light of empirical considerations, the latter
two models are based upon de.ned physical approaches depicting interaction between light and matter.
The theoretical basis of these methods, the validation methodologies used to support them and the
similarity in the spectra corrected by light re-absorption for both models, all showed that they should be
treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.
two models are based upon de.ned physical approaches depicting interaction between light and matter.
The theoretical basis of these methods, the validation methodologies used to support them and the
similarity in the spectra corrected by light re-absorption for both models, all showed that they should be
treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.
F685/F737 2). While the .rst method was developed in the light of empirical considerations, the latter
two models are based upon de.ned physical approaches depicting interaction between light and matter.
The theoretical basis of these methods, the validation methodologies used to support them and the
similarity in the spectra corrected by light re-absorption for both models, all showed that they should be
treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.