INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Re-absorption of chlorophyll fluorescence in leaves revisited. A comparison of correction models
Autor/es:
GABRIELA B. CORDON AND M. GABRIELA LAGORIO
Revista:
Photochemical and Photobiological Sciences
Editorial:
RSCPublishing
Referencias:
Lugar: Cambridge; Año: 2006 vol. 5 p. 735 - 735
ISSN:
1474-905X
Resumen:
The application of correction methods to account for re-absorption of chlorophyll .uorescence emission in leaves is subject to a number of controversies in the literature. These uncertainties lead to high discrepancies in the corrected spectral distribution of .uorescence and consequently in the interpretation of related physiological features of plants, according to the chosen method used in the process of correction. In this research, three correction methods, based on transmittance and/or re.ectance measurements on leaves, were analysed comparatively. One method gave high values for the corrected .uorescence ratio between 685 nm and 737 nm (F685/F737 ˜ 7 to 20 according to the different species of leaves). The two other methods were found to give similar results with corrected .uorescence ratios around a value of two (F685/F737 ˜ 2). While the .rst method was developed in the light of empirical considerations, the latter two models are based upon de.ned physical approaches depicting interaction between light and matter. The theoretical basis of these methods, the validation methodologies used to support them and the similarity in the spectra corrected by light re-absorption for both models, all showed that they should be treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves. two models are based upon de.ned physical approaches depicting interaction between light and matter. The theoretical basis of these methods, the validation methodologies used to support them and the similarity in the spectra corrected by light re-absorption for both models, all showed that they should be treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves. other methods were found to give similar results with corrected .uorescence ratios around a value of two (F685/F737 ˜ 2). While the .rst method was developed in the light of empirical considerations, the latter two models are based upon de.ned physical approaches depicting interaction between light and matter. The theoretical basis of these methods, the validation methodologies used to support them and the similarity in the spectra corrected by light re-absorption for both models, all showed that they should be treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves. two models are based upon de.ned physical approaches depicting interaction between light and matter. The theoretical basis of these methods, the validation methodologies used to support them and the similarity in the spectra corrected by light re-absorption for both models, all showed that they should be treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves. F685/F737 ˜ 7 to 20 according to the different species of leaves). The two other methods were found to give similar results with corrected .uorescence ratios around a value of two (F685/F737 ˜ 2). While the .rst method was developed in the light of empirical considerations, the latter two models are based upon de.ned physical approaches depicting interaction between light and matter. The theoretical basis of these methods, the validation methodologies used to support them and the similarity in the spectra corrected by light re-absorption for both models, all showed that they should be treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves. two models are based upon de.ned physical approaches depicting interaction between light and matter. The theoretical basis of these methods, the validation methodologies used to support them and the similarity in the spectra corrected by light re-absorption for both models, all showed that they should be treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves. F685/F737 ˜ 2). While the .rst method was developed in the light of empirical considerations, the latter two models are based upon de.ned physical approaches depicting interaction between light and matter. The theoretical basis of these methods, the validation methodologies used to support them and the similarity in the spectra corrected by light re-absorption for both models, all showed that they should be treated as con.dent and suitable approximations to solve the problem of light re-absorption in leaves.