INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
FI-SPE-ETAAS determination of Cr(VI) by selective separation and peconcentration on a lab-made hybrid mesoporous solid microcolumn
MANUELA KIM; JORGE STRIPEIKIS; MABEL TUDINO
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY
Año: 2009 vol. 64 p. 500 - 500
A lab-made hybrid mesoporous solid was employed in a solid phase flow injection system coupled to an electrothermal atomizer (FI-SPE-ETAAS) for the selective retention of Cr(VI). The solid was prepared by co-condensation of sodium tetraethylortosilicate and 3-aminopropyltriethoxysilane by sol-gel methodology and one-pot synthesis and characterized by IR, XRD, and SEM. Adsorption capacities at different pH values of both, Cr(VI) and Cr(III), were also measured in order to obtain the optimum retention for Cr(VI) with no interference of Cr(III). The maximum capacity of adsorption (4.35 mmol g-1) was observed for pH values between 2-3, whilst Cr (III) was found to remain in solution (adsorption capacity= 0.007 mmol g-1). Then, a microcolumn (bed volume: 7.9 µL) was filled with the solid and inserted in the FI-ETAAS system for analytical purposes. Since the analyte was strongly retained by the filling in the anionic form, hydroxilammonium chloride 1.0.10-4 mol L-1 in hydrochloric acid 1 mol L-1 was selected as eluent due to its redox characteristics. In this way, the sorbed Cr(VI) was easily released in the cationic form. The enrichment factor (EF) was found as a compromise between sensitivity and sample throughput and a value of 27 was obtained under optimized conditions: pH 2, sample loading 2 mL min-1 (60 seconds), elution flow rate 1 ml min-1 (eluent volume: 75ìL). Under optimized conditions the limit of detection for Cr(VI) was 1.17 ng L-1, RSD% of 2.5%, sample throughput of 21 per hour, microcolumn lifetime over 300 adsorption/desorption cycles. Cr(III) determination was also performed by simply measuring its concentration at the end of the column and after Cr(VI) retention by the mesoporous solid. Applications of the methodology to the determination of Cr(VI) in river, osmosis, tap, mineral and effluent waters showed excellent results. Validation was performed by means of recovery studies as no certified materials were available for Cr(VI). Total chromium determinations, obtained by the sum of Cr(III) and Cr(VI) concentrations, were validated using NIST, SRM 1643e certificate reference material (Trace Element in Natural Water).