INQUIMAE 12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Mapping the protein-binding sites for iridium(III)-based CO-releasing molecules
Autor/es:
A. VERGARA; F. DOCTOROVICH; A.A. PETRUK; D. MARASCO; M. CATERINO; G. FERRARO; A. MERLINO; D.A. ESTRIN
Revista:
DALTON TRANSACTIONS
Editorial:
ROYAL SOC CHEMISTRY
Referencias:
Lugar: CAMBRIDGE; Año: 2016 vol. 45 p. 12206 - 12214
ISSN:
1477-9226
Resumen:
A combination of mass spectrometry, Raman microspectroscopy, circular dichroism and X-ray crystallography has been used to obtain detailed information on the reaction of an iridium-based CO-releasing molecule (Ir-CORM), Cs2IrCl5CO, with a model protein, bovine pancreatic ribonuclease. The results show that Ir-compound fragments bind to the N-terminal amine and close to histidine and methionine side chains, and the CO ligand is retained for a long time. The data provide helpful information for identifying protein targets for Ir-CORMs and for studying the mechanism that allows them to exhibit their interesting biological properties.
