INQUIMAE 12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Quantum Dots As Ultrasensitive Nanoactuators and Sensors of Amyloid Aggregation in Live Cells
Autor/es:
M. JULIA ROBERTI; M. MORGAN; G. MENÉNDEZ; L. PIETRASANTA; THOMAS M. JOVIN; E. A. JARES-ERIJMAN
Revista:
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Referencias:
Año: 2009 vol. 131 p. 8102 - 8107
ISSN:
0002-7863
Resumen:
Quantum dots multifunctionalized with the amyloid protein α-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar−millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinsons disease, is accelerated up to 15-fold with only 100 nanoparticles. The combination with a tetracysteine-tagged form of α-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells.
