INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Multiphoton Excitation of Upconverting Nanoparticles in Pulsed Regime
Autor/es:
ZHIJUN CHEN; SI WU; JOSE HODAK; ROBERTO ETCHENIQUE
Revista:
ANALYTICAL CHEMISTRY
Editorial:
AMER CHEMICAL SOC
Referencias:
Lugar: Washington; Año: 2016 vol. 88 p. 1468 - 1468
ISSN:
0003-2700
Resumen:
Upconverting nanoparticles (UCNPs) presentemission in the visible region upon irradiation with NIR lightthrough a multiphoton mechanism. However, the longcharacteristic time of their emission has prevented the use ofthis kind of entities as multiphoton probes. We present a studyon the use of erbium-containing UCNPs under pulsedexcitation, showing that both the power density and theduration of the excitation pulse are key factors to understandthe emission behavior. By adjusting power and excitation rate,we can obtain typical multiphoton z-axis focal exclusiveexcitation. These findings open the possibility of using UCNPsas probes for controlled localization of uncaging and imagingwith multiphoton z-axis sectioning. We show that this can beachieved even at power densities several orders of magnitude lower than traditional multiphoton microscopies.