INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Nanoscale separation of molecular species based on their rotational mobility
Autor/es:
ILARIA TESTA; ANDREAS SCHĂ–NLE; CLAAS V. MIDDENDORFF; CLAUDIA GEISLER; REBECCA MEDDA; CHRISTIAN A. WURM; ANDRE C. STIEL; STEFAN JAKOBS; MARIANO BOSSI; CHRISTIAN EGGELING; STEFAN W. HELL; ALEXANDER EGNER
Revista:
OPTICS EXPRESS
Referencias:
Año: 2008 vol. 16 p. 21093 - 21093
ISSN:
1094-4087
Resumen:
We combine far-field fluorescence nanoscopy through serializedrecording of switchable emitters with polarization-sensitive fluorescencedetection. In addition to imaging with nanoscale spatial resolution, thistechnique allows determination of the fluorescence anisotropy of eachdetected dipole emitter and thus an estimate of its rotational mobility. Subpopulationsof fluorescent markers can thus be separated based on theirinteraction with the sample. We applied this new functional nanoscopy toimaging of living mammalian cells.