INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Fluorescence nanoscopy by ground-state depletion and single-molecule return
Autor/es:
JONAS FĂ–LLING; MARIANO BOSSI; HANNES BOCK; REBECCA MEDDA; CHRISTIAN A WURM; BIRKA HEIN; STEFAN JAKOBS; CHRISTIAN EGGELING; STEFAN W HELL
Revista:
Nature Methods
Referencias:
Año: 2008 vol. 5 p. 943 - 943
Resumen:
We introduce far-field fluorescence nanoscopy with ordinaryfluorophores based on switching the majority of them to ametastable dark state, such as the triplet, and calculating theposition of those left or those that spontaneously returned to theground state. Continuous widefield illumination by a single laserand a continuously operating camera yielded dual-color imagesof rhodamine- and fluorescent protein–labeled (living) samples,proving a simple yet powerful super-resolution approach.