Multicolor Far-Field Fluorescente Nanoscopy through Isolated Detection of Distinct Molecular Species

MARIANO BOSSI; JONAS FÖLLING; VLADIMIR N. BELOV; VADIM P. BOYARSKIY; REBECCA MEDDA; ALEXANDER EGNER; CHRISTIAN EGGELING; ANDREAS SCHÖNLE; STEFAN W. HELL

NANO LETTERS (PRINT)

American Chemical Society

Año: 2008 vol. 8 p. 2463 - 2468

1530-6984

By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstratesimultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. Theapplicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopywith the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.