INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
Electron transfer in SAM/Cytochrome/Polyelectrolyte hybrid systems on electrodes: A time-resolved surface-enhanced resonance Raman study
Autor/es:
GROCHOL J (GROCHOL, JANA), DRONOV R (DRONOV, ROMAN), LISDAT F (LISDAT, FRED), HILDEBRANDT P (HILDEBRANDT, PETER), MURGIDA DH (MURGIDA, DANIEL H.)
Revista:
LANGMUIR
Referencias:
Año: 2007 vol. 23 p. 11289 - 11294
ISSN:
0743-7463
Resumen:
Silver electrodes were covered with mixed self-assembled monolayers
(SAMs) of 11-mercaptoundecanoic acid (MUA) and 11-mercaptoundecanol
(MU) and subsequently coated with alternating layers of cytochrome c
(Cyt) and poly(anilinesulfonic acid) (PASA). The immobilized protein is
electroactive and retains its native structure. Compared to the case of
systems on gold electrodes, the stability of the assembly was found to
be decreased. The redox process of Cyt is accompanied by reversible
oxidation-reduction of PASA as revealed by the comparative
surface-enhanced resonance Raman (SERR) analysis of assemblies
including Cyt and the redox-inactive apo-cytochrome c. Timeresolved
SERR experiments show a fast electron exchange between the protein and
the polyelectrolyte that may play a supporting role in the electric
communication of thicker multilayer assemblies employed as sensors.