INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
Chemical Two-Photon Fluorescence
GUILLERMO CARRONE; ROBERTO ETCHENIQUE
AMER CHEMICAL SOC
Lugar: Washington; Año: 2015 vol. 87 p. 4363 - 4363
We describe a method based on a cagedfluorescent molecule that can act as a chemical two-photonprobe. It is composed of an organic fluorophore and aruthenium−bipyridine complex that acts as a photoremovablequencher. For the fluorophore to be emissive, two independentphotons must act on the molecule: the first photon frees thefluorescent ligand from the Ru complex and the second photonexcites the fluorescence. In this two-photon regime, theemission is not proportional to the excitation intensity butrather to its second power, as in traditional two-photonsystems based on ultrashort pulsed high-power lasers. Thisquadratic relationship implies a much higher spatial precisionon the z-axis when the probe is used in a microscopytechnique. The chemical nature of the two-photon excitationmechanism allows the use of inexpensive low-power lasers.