Circannual rhythms of Acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus

MENENDEZ HELMAN R. J.; FERREYROA, G.V.; DOS SANTOS AFONSO, M.; SALIBIÁN, A

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Lugar: Amsterdam; Año: 2015 vol. 111 p. 236 - 241

0147-6513

The use of biomarkers as a tool to assess responses of organisms exposed to pollutants in toxicity bioassays, as well as in aquatic environmental risk assessment protocols, requires the understanding of the natural fluctuation of the particular biomarker. The aim of this study was to evaluate the intrinsic variations of acetylcholinesterase (AChE) activity in tissues of a native freshwater teleost fish, Cnesterodon decemmaculatus, collected at different seasons, to be used as biomarker in toxicity tests. Specific AChE activity was measured by the method of Ellman in homogenates of fish anterior section finding a seasonal variability. The highest activity was observed in summer, decreasing significantly below 40% in winter. The annual AChE activity cycle in the anterior section was fitted to a sinusoidal function with a period of 11.2 months. Moreover, an inverse relationship between enzymatic activity and the animal size was established. The results showed that both the fish length and seasonal variability affect AChE activity. AChE activity in fish posterior section showed a similar trend to that in the anterior section, while seasonal variations of the activity in midsection were observed but differences were not statistically significant.   In addition, no relationship between AChE and total tissue protein was established in the anterior and posterior sections suggesting that the circannual rhythms observed are AChE-specific responses. Results highlight the importance of considering both the fish size and season variations to reach valid conclusions when AChE activity is employed as neurotoxicity biomarker. cant.