INQUIMAE   12526
INSTITUTO DE QUIMICA, FISICA DE LOS MATERIALES, MEDIOAMBIENTE Y ENERGIA
Unidad Ejecutora - UE
artículos
Título:
“Structural Characterization of Native High-Methoxylated Pectin using NMR Spectroscopy and UV-MALDI-TOF Mass Spectrometry. Comparative use of 2,5-Dihydroxybenzoic Acid and n-Harmane as UV-MALDI Matrices"
Autor/es:
MONGE, M.E.; R. M. NEGRI, A.A. KOLENDER, R. ERRA-BALSELLS.
Revista:
RAPID COMMUNICATIONS IN MASS SPECTROMETRY : RCM.
Referencias:
Año: 2007 vol. 21 p. 2638 - 2638
ISSN:
0951-4198
Resumen:
The successful analysis by ultraviolet matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (UV-MALDI-TOF MS) of native and hydrolyzed high-methoxylated pectin samples is described. In order to find the optimal conditions for UV-MALDI-TOF MS analysis several experimental variables were studied such as: different UV-MALDI matrices (nor-harmane, 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. nor-harmane, 2,5-dihydroxybenzoic acid), sample preparation methods (mixture, sandwich), inorganic salt addition (doping salts, NaCl, KCl, NH4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. 4Cl), ion mode (positive, negative), linear and reflectron mode, etc. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. nor-Harmane has never been used as a UV-MALDI matrix for the analysis of pectins but its use avoids pre-treatment of the sample, such as an enzymatic digestion or an acid hydrolysis, and there is no need to add salts, making the analysis easier and faster. This study suggested an alternative way of analyzing native high-methoxylated pectins, with UV-MALDI-TOFMS, by using nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. nor-harmane as the matrix in negative ion mode. The analysis by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described. spectroscopy of the native and hydrolyzed pectin is also briefly described. 1H and 13C nuclear magnetic resonance (NMR) spectroscopy of the native and hydrolyzed pectin is also briefly described.